Laboratoire d'endocrinologie circadienne

Responsable: Dre Charna DIBNER 

Dr. Charna Dibner Division of Endocrinology, Diabetes, Hypertension and Nutrition Département Spécialités de Médicine  Department of Cell Physiology and Metabolism Faculty of Medicine, University of Geneva  Address:  Centre Médical Universitaire D05.2147a  Dibner Group, PHYM Rue Michel-Servet, 1 CH-1211 Geneva 4, Switzerland Phone:  +41 22 3795934 Email: Charna.Dibner@hcuge.ch

  • Division of Endocrinology, Diabetes, Hypertension and Nutrition
  • Département Spécialités de Médicine
  • Department of Cell Physiology and Metabolism
  • Faculty of Medicine, University of Geneva
Address: 
Centre Médical Universitaire D05.2147a 
Dibner Group, PHYM
Rue Michel-Servet, 1
CH-1211 Geneva 4, Switzerland
Phone:  +41 22 3795934
Email: Charna.Dibner@hcuge.ch

 

Activités de recherche

Le but de la recherche menée par ce groupe est d'explorer le lien entre les horloges circadiennes mammifères et le métabolisme. L'équipe de recherche s'intéresse en particulier à l'importance physiologique d'une horloge pancréatique et son rôle dans les maladies métaboliques et le diabète de type 2 au travers de trois projets:

  1. Rôle des horloges cellulaires sur la fonction des cellules béta pancréatiques: l’enregistrement de la bioluminescence dans les cellules vivantes est employé pour analyser l’expression des gènes et leur fonction dans des conditions physiologiques et de rythme circadien aboli
  2. Rôle des horloges cellulaires de la souris sur l’homéostasie du glucose: l’enregistrement de la bioluminescence en temps réel permet d’étudier la contribution des horloges biologiques à la sécrétion d’insuline et l’homéostasie du glucose dans des conditions physiologiques et de diabète de type 2
  3. Etude des horloges circadiennes humaines: à travers les rythmes circadiens de cellules provenant de biopsies cutanées, la fonction des horloges est comparée chez des sujets en bonne santé et diabétiques

Group members:

Past group members:

  • Dr. Anne-Marie Makhlouf, former PhD student, currently senior collaborator in Nutrition Unit, HUG
  • Dr. Camille Saini, postdoc in our lab, currently Clinical Coordinator, Epidemiology Unit, HUG
  • Dr. Laurianne Giovannoni, scientific collaborator in our lab, currently MD at Paediatric Service, HUG
  • Dr. Zhanna Chitikova, guest scientist in our lab (volunteer), currently postdoc at Dpt of Molecular Biology, Faculty of Science, University of Geneva  
  • Dr. Pamela Pulimeno, postdoc in our lab, currently senior postdoc at Harvard Medical School, Boston, US

Selected recent publications by the group:

2017 - Original articles

Jacovetti C., Rodriguez A., Gattesco S., Petrenko V., Saini C., Dibner C., Regazzi R. “MicroRNAs modulate core-clock gene expression in pancreatic islets during early postnatal lifes.” Research article, Diabetologia IF 6.487

Chanon S., Durand C., Vieille-Marchiset A., Robert M., Dibner C., Simon C., Lefai E. “Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes.” J Vis Exp IF 1.1

3. Petrenko V., Gosmain Y., and Dibner C. “High-resolution recording of circadian oscillator features in primary cultures of mouse a- and b-cells.” Original article, accepted for publication. Frontiers in Endocrinology IF: to be attributed in 2017

4. Petrenko V., Saini C., Giovannoni L., Gobet C., Sage D., Unser M., Heddad-Mason M., Gu G., Bosco D., Gachon F., Philippe J., Dibner C. “Pancreatic a- and b-cellular clocks impact differentially on hormone secretion and gene transcription due to their distinct molecular properties.” Genes and Development 2017, Feb 15;31(4):383-398. IF 11.7, 6 citations

Research highlight on this article: Haspel J. “Clocks stop sugar shock” Science Translational Medicine

Review articles

Petrenko V. and Dibner C. “Circadian orchestration of insulin and glucagon release.” Invited featured article, accepted for publication in Cell Cycle IF 4.56

Gachon F., Loizides-Mangold U., Petrenko V. and Dibner C. “Glucose homeostasis: regulation by peripheral circadian clocks in rodents and humans.” Endocrinology 2017, Mar 15. doi: 10.1210/en.2017-00218. IF 4.2

Dibner C., Sadowski S., Triponez F. and Philippe J. “The search for preoperative biomarkers for thyroid carcinoma: application of the thyroid circadian clock properties” Invited perspective article, accepted for publication. Biomark Med. 2017, Mar;11(3):285-293 IF 2.3

2016 - Original articles

Petrenko V., Saini C., Perrin L., Dibner C. “Parallel measurement of circadian clock gene expression and hormone secretion in human primary cell cultures.” J Vis Exp., 2016, Nov 11;(117). IF1.1, 2 citations

Makhlouf A.-M., Chitikova Z., Pusztaszeri M., Bercy M., Delucinge-Vivier C., Triponez F., Meyer P., Philippe J., and Dibner C. “Identification of CHEK1, SLC26A4, c-KIT, TPO and TG as new biomarkers for human follicular thyroid carcinoma diagnostics” Oncotarget 2016, Jun 18. doi: 10.18632. IF 6.37, 6 citations

Loizides-Mangold U., Koren-Gluzer M., Skarupelova S., Makhlouf A.-M., Hayek T., Aviram M., Dibner C. “Paraoxonase 1 (PON1) and pomegranate influence circadian gene expression and period length.” Chronobiology International, IF 3.3, 1 citation

Saini C., Petrenko V., Pulimeno P., Giovannoni L., Berney T., Hebrok M., Howald C., Dermitzakis E., and Dibner C. “A functional circadian clock is required for proper insulin secretion by human pancreatic islet cells.” Diabetes, Obesity and Metabolism 2016 Apr;18(4):355-65. IF 6.4, 15 citations

PhD Thesis Defense

Dr. Anne-Marie-Makhlouf

2015 - Original articles

Perrin L., Loizides-Mangold U., Skarupelova S., Pulimeno P., Chanon S., Robert M., Bouzakri K., Moudoux C., Roux-Lombard P., Vidal H., Lefai E. and Dibner C. “Human skeletal myotubes display a cell-autonomous circadian clock implicated in basal myokine secretion.” Molecular Metabolism 2015, 4 (11), 834-845. IF 5.5, 25 citations

Chitikova Z., Pusztaszeri M., Makhlouf A.-M., Bercy M., Delucinge-Vivier C., Triponez F., Meyer P., Philippe J., and Dibner C.  “Identification of new biomarkers for human papillary thyroid carcinoma employing NanoString analysis.” Oncotarget 2015 May 10;6(13):10978-93. IF 6.4, 12 citations

Review articles

Dibner C. and Schibler U. “A pancreatic clock times insulin release” Science 2015, 350 (6261), 628-629. IF 33.6, 5 citations

Dibner C. and Gachon F. “Circadian dysfunction and obesity: is Leptin the missing link?” Cell Metabolism 2015 Vol. 22(3) 359-360. IF 17.6

Saini C., Brown S. and Dibner C. “Skin fibroblast clocks: applications to studying human circadian phenotypes in physiology and pathophysiology.”  Frontiers in Neurology 2015 May 13;6:95. IF 3.6, 28 citations  

Dibner C., Schibler U. “Circadian clocks: roles in endocrinology and metabolism” Journal of Internal Medicine 2015, IF 6.5, 80 citations

Philippe J. and Dibner C. “Thyroid circadian timing: roles in physiology and thyroid malignancies” J Biol Rhythms 2015 Apr;30(2):76-83. IF 3.3, 15 citations.

Related video and links:

ECM Video 1 and 3

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3563957/

http://www.jove.com/video/54673/parallel-measurement-circadian-clock-gen...

http://bigwww.epfl.ch/sage/soft/circadian/

Téléchargez le résumé des recherches menées dans le laboratoire d'endocrinologie circadienne (en anglais)

 

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